Participant: PROMISE AGEP Research Symposium
Denise N. Williams
Department: Chemistry and Biochemistry
Institution: University of Maryland Baltimore County (UMBC)
Nontraditional Antibacterials: Combining Antibacterial Entities into One Effective Treatment
Denise N. Williams1, Julia Saar2, Vera Bleicher2,3, Sibylle Rau3, Karen Lienkamp2,4, Zeev Rosenzweig1
1Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD
2Department of Microsystems Engineering (IMTEK), Albert-Ludwigs-Universität, Freiburg, Germany
3Department of Operative Dentistry and Periodontology, Center for Dental Medicine of the Albert-Ludwigs-Universität, Freiburg, Germany
4Freiburg Institute for Advanced Studies (FRIAS), Albert-Ludwigs-Universität, Freiburg, Germany
This presentation will review ongoing research aimed to validate the hypothesis that conjugating an antimicrobial polymer to an antibacterial nanoparticle will be able to synergistically increase their activity against harmful bacterial cell growth. The polymers, butyl poly(oxonorborene)s or PONs, are antimicrobial polymers with selective, tunable, broad-spectrum activity against bacteria; including against some bacterial strains for which we are observing increasing cases of multidrug resistance. Previous work has shown free PONs conjugated to silicon wafers, gold wafers, polymer matrices, or gold nanoparticles can improve the activity of the polymers against bacterial targets. Concurrently, research into the antibacterial properties of CdTe quantum dots (QD), a luminescent nanoparticle, has identified this material to have light-activated antibacterial activity. Hence, in this study we conjugated the series of PONs to CdTe QD and then compared the conjugates’ activity against Escherichia coli, Staphylococcus aureus, and human red blood cells to the activity of the free PONs series and CdTe QD. Compared to the free PONs, the PONs-QD conjugates had stronger activity against the growth of E. coli, but lesser activity against S. aureus and human red blood cells. The strong activity against E. coli combined with the minimal activity against red blood cells illuminate the potential for these conjugates to be used for the desired antibacterial applications, and with a smaller concern for off-target effects than we would have with free PONs application.
Future Antibiotics? Interactions between Quantum Dots and Bacterial Cells
Quantum dots are luminescent semiconductor nanoparticles with unique optical and chemical properties. These properties have found purpose in technologies of several different fields of science, including energy harvesting, computing, chemistry, and biology. Specifically considering biological implications, QDs can be found in luminescent probes, cancer sensors, and constructs for antibiotic purposes. Essential to the use of QDs in biology is the understanding of how QDs interact with their environment. Research to this end has focused on unintended interactions between QDs and bacteria as their size and metal components have known harmful impacts on bacteria viability, but scientists are beginning to research how to turn unintended and harmful interactions into targeted ones. This talk will review the concept of turning cell-penetrating interactions between QDs and bacteria into targeted treatments specifically against pathogenic bacteria.
Synthesis and Environmental Studies of CdSe/ZnS and ZnSe/ZnS Quantum Dots
Denise Williams1, Sunipa Pramanik2, Christy Haynes2, and Zeev Rosenzweig1
- University of Maryland Baltimore County, Department of Chemistry and Biochemistry, Baltimore, MD 21250
- University of Minnesota, Department of Chemistry, Minneapolis, MN 55455
This presentation describes the synthesis, characterization, and environmental impact of luminescent CdSe/ZnS and ZnSe/ZnS quantum dots (QD). Cadmium-containing QD, such as CdSe/ZnS, are commonly used because of their superior optical properties in comparison to other material QD. More recently though, cadmium-free alternatives, such as ZnSe/ZnS QD, are becoming more attractive because they lack this known toxic element which should inevitable reduce the environmental impact of QD. The synthesis of the CdSe/ZnS and ZnSe/ZnS systems are similar colloidal processes, differing only in the use of dopants for the ZnSe/ZnS QD to cause the emission profile to mirror that of the CdSe/ZnS QD. The material characterization of these QD involves analysis of brightness, polydispersity, lifetime, chemical stability, and photostability using absorbance, fluorescence, and mass spectrometry techniques. With the anticipation that cadmium-free QD may replace cadmium-containing QDs in a wide range of applications, the environmental impact of the two systems is modeled in this study with the organism Shewanella oneidensis MR-1. These studies have validated the hypothesis that the ZnSe/ZnS QD can be significantly less toxic than their cadmium alternative, while allowing for an optically comparable technology.
Denise N. Williams is a PhD candidate at the University of Maryland, Baltimore County (UMBC) as a member of Dr. Zeev Rosenzweig’s research group. With this group, she studies the synthesis, characterization, human health and environmental impact of optically active quantum dots. Denise is currently a National Science Foundation AGEP Fellow, a Meyerhoff Graduate Fellow, and a research member of the Center for Sustainable Nanotechnology. Denise is also active on campus as a senator for the UMBC Chemistry Graduate Student Association and secretary for the Living Network of Connective Sciences. Prior to her time at UMBC, Denise earned a Bachelor of Science in Chemistry and a Bachelor of Science in Forensic Science from the University of New Haven in West Haven, Connecticut in May 2015.
GENERAL SUMMARY OF GRADUATE RESEARCH
Denise Williams’ current research focuses on the characterization of luminescent CdSe, CdTe, ZnSe, and InP quantum dots (QDs) in an effort to replace toxic cadmium-containing nanomaterials with sustainable materials of similar optical capabilities. She is comparing the chemical properties, optical properties and toxic impacts of ZnSe, CdSe, and CdTe QDs against model liposomes, bacteria, and nematodes. Thus far, a clear difference in the interactions these QDs have with their environment is evident and being investigated on a molecular level.
Beyond comparing the material implications on QD interactions, the affect various surface coatings have on interactions between QDs and bacteria is under investigation. Molecular, polymer, and peptide coatings have been investigated in the field to deter and to cause specific interactions between nanoparticles and bacteria. Denise’s research aims to add unique polymer and peptide coatings to the surface of cadmium-free QDs in order to specifically target pathogenic bacteria over bacteria essential for human and environmental health.
SELECTED LIST OF PRESENTATIONS AND PUBLICATIONS
- Williams, D. N.; Pramanik, S.; Brown, R. P.; Zhi, B.; McIntire, E.; Hudson-Smith, N.V.; Haynes, C.; Rosenzweig, Z. Adverse Interaction of Luminescent Semiconductor Quantum Dots with Liposomes and Shewanella oneidensis. ACS Applied Nano Materials. 2018, 1 (9), 4788–4800.
- Zhi, B.; Cui, Y.; Wang, S.; Frank, B.; Williams, D. N.; Brown, R.; Melby, E.; Hamers, R.; Rosenzweig, Z.; Fairbrother, D. H.; Orr, G.; Haynes, C. Malic acid carbon dots: from super-resolution living cell imaging to highly efficient separation. ACS Nano. 2018, 12(6), 5741-5752.
- Williams, D. (2017, March). Bacterial impact studies of CdSe quantum dots versus ZnSe quantum dots. Three Minute Thesis competition presentation at UMBC Graduate Research Conference. First place presentation.
- Pham, S.; Kuether, J.; Gallagher, M.; Hernandez, R.; Williams, D.; Zhi, B.; Mensch, A.; Hamers, R.; Rosenzweig, Z.; Fairbrother, D. Howard; Krause, M.; Feng, V. Z, ; Haynes, C. Carbon Dots: A modular activity to teach fluorescence and nanotechnology at multiple levels. Chem. Edu. 2017, 94 (8), 1143–1149.
- Lyons, T. Y.; Williams, D. N.; Rosenzweig, Z. Addition of Fluorescence Lifetime Spectroscopy to the Tool Kit Used to Study the Formation and Degradation of Luminescent Quantum Dots in Solution. Langmuir. 2017, 33 (12), 3018-3027.
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