Brianda Beverley

Participant: PROMISE AGEP Research Symposium

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Brianda Beverley

Department: Biological Sciences

Institution: University of Maryland, Baltimore County (UMBC)

 

2018 ABSTRACT

Translation in Saccharomyces cerevisiae

Translation is very efficient in Saccharomyces cerevisiae. In fact, translation codon errors vary from 4.0×10-5 to 6.9×10-4 per codon in this yeast species. It is hypothesized that the regulation of errors is controlled by Protein Kinase C 1 (Pkc1) via the help of a scaffolding protein Asc1. To observe the effects of Pkc1, a knockout of the Asc1 gene was performed in two strains: wild-type and ∆Ctk1. C-terminal Domain kinase 1 (Ctk1) phosphorylates the ribosomal protein Rps2 which increases translation efficiency. A decrease in translation efficiency has been observed when Ctk1 is depleted. The double knockout, ∆Ctk1Asc1, is hypothesized to have an increase in translation efficiency. After successful knockout of the Asc1 gene, the amount of errors will be quantified by a beta-galactosidase reporter gene system that carries a mutation at the 537 amino acid site. The frequency of errors can be calculated by comparing beta-galactosidase activity of the wild-type and mutant strains. Figuring out the details of protein synthesis will not only help to further the understanding of translation efficiency, but also the information gained can be used to make protein synthesis more efficient for biotech companies.

Brianda Beverley is a Biological Sciences Master’s degree student at the University of Maryland Baltimore County. She received her Bachelor’s degree in Biology from George Mason University in Fairfax, Virginia. Upon graduation, Brianda was accepted into and completed a Medical Laboratory Science certification program at INOVA Fairfax hospital. Her research interests include microorganisms involved in infectious diseases.

 

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